Dna strawberry conclusion

Sir Dennis Macapagal Background of the Study: The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such as insulin, antibiotics, and hormones.

Dna strawberry conclusion

Experimental Procedure Chill the rubbing alcohol in the freezer. You will need it later. Set the mixture aside. This is your extraction liquid. Line a funnel with the cheesecloth. Remove the green stems from three strawberries, put the strawberries in a plastic, re-sealable sandwich bag, and push out all the extra air.

Seal the bag tightly. With your fingers, squeeze and smash the strawberries for 2 minutes. After this the strawberries should look like Figure 3 below.

After smashing the strawberries for two minutes, they should look like the ones in this image. Push out all the extra air and reseal the bag.

The liquid detergent will help break the strawberry cells open, allowing the DNA to spill out.

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The salt helps create an environment where the different strands of DNA can gather together in a clump, making it easier for you to see them.

Squeeze the strawberry mixture with your fingers for 1 minute. Do you notice any differences in the mixture after mixing it for 1 minute? After this the strawberries should look like Figure 4 below. After smashing the strawberries with the extraction liquid for one minute, the mixture should look like the one in this image.

Pour the strawberry mixture from the bag into the funnel. Let it drip into the glass until there is very little liquid left in the funnel i.

You may need to be patient as it can take some time for the liquid to filter through the cheesecloth. Be sure not to let any pulp go around the cheesecloth and end up in the drinking glass below.

Dna strawberry conclusion

If this happens, you will need to pour the liquid through the cheesecloth again, into a new drinking glass. Throw away the cheesecloth and the strawberry pulp inside. The alcohol should form approximately a one-inch deep layer on top of the strawberry liquid.

Dna strawberry conclusion

If you have a small test tube or jar you will not need all of the alcohol. Do not let the alcohol and strawberry liquid mix. The DNA collects between the two layers. DNA does not dissolve in alcohol.

When alcohol is added to the mixture, the rest of the mixture, except for the DNA, stays in solution, while the DNA precipitates out into the alcohol layer. Study the mixture inside of the test tube or jar. Do you see anything in the jar that might be strawberry DNA? If so, where in the jar is it?

Record your observations in your lab notebook. You can collect the DNA with a skewer or other thin rod. Dip the bamboo skewer into the test tube to where the alcohol and strawberry layers meet.

Pull up the skewer. You should find some whitish, stringy stuff on the skewer, which is DNA containing strawberry genes! If you like this project, you might enjoy exploring these related careers:Strawberry DNA Conclusion The purpose of this lab was to extract DNA from strawberries.

When we put rubbing alcohol into the mixture of extracted strawberry juice, dish soap, and . After you added the cold rubbing alcohol to the filtered strawberry liquid, the alcohol should have precipitated the DNA out of the liquid while the rest of the liquid remained in solution.

Experiment 6 DNA Extraction from Strawberries Submitted by: Mae Abeguel L. Hapal BS IOP Submitted to: Sir Dennis Macapagal Background of the Study: Since DNA is the blueprint for life, everything living contains DNA.

The extraction buffer helps to release the DNA from the surrounding cell components of the crushed strawberry.

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3. What does the filter do? The filter removes the larger particles from the solution, such as seeds, pith, etc., allowing only the smaller cell components such as the DNA, proteins, etc. to filter through. Questions Parts II and III: 1. Oct 19,  · The extraction of DNA was facilitated by the presence of eight copies of each chromosome, or an octoploid genome, in the strawberry.

In contrast, humans have a diploid genome, which includes only two copies of each chromosome. If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture.

Variables: The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of DNA extracted.

Conclusion - Strawberry DNA Extraction- Noah Mathews